Pdf point mutation impairs centromeric cenh3 loading and. Haploid production by centromere mediated genome elimination 1. Centromere functional defects underlie genome elimination in barley. We have also exploited centromeremediated genome elimination to convert a natural tetraploid arabidopsis into a diploid, reducing its ploidy to simplify breeding. We crossed gfptailswap to the natural tetraploid warschau1 wa1, and althoughover98%ofseedswereaborted,viablef 1 progenyincluded. Simon chan 19742012 simon started the cenh3 project when he joined ucd in 2006. Mechanistic hypotheses to explain uniparental genome elimination.
Epigenetic differences between parents can trigger uniparental centromere failure and genome elimination, contributing to postzygotic hybridization barriers. Nov 07, 2017 haploid arabidopsis thaliana produced by crossing plants expressing altered cenh3 to wild type. Production of haploid plants that inherit chromosomes from only one parent can greatly accelerate plant breeding. In plants, centromere mediated genome elimination is used to 361 produce maternal haploids kelliher et al.
Uniparental genome elimination is a widespread outcome of distant genetic crosses. Haploid plants produced by centromeremediated genome elimination. Mar 25, 2010 the fact that haploids were produced with both gfptailswap and gfpcenh3 transgenes indicates that multiple different alterations to the protein may induce genome elimination in other plants. As cenh3 is universal in eukaryotes, our method may be extended to produce haploids in any plant species. Production of haploid plants that inherit chromosomes from only one parent can greatly accelerate plant breeding1, 2, 3. Chanhaploid plants produced by centromeremediated genome elimination.
Mar 25, 2010 haploid plants produced by centromere mediated genome elimination. Current knowledge of cenh3mediated uniparental letters nature plants genome elimination should allow for the creation of maternal haploid inducers in a wider range of species 30. His laboratory achieved a lot in the 6 years that followed. Haploid induction by centromere mediated genome elimination 3.
In plants, centromeremediated genome elimination is used to 361 produce maternal haploids kelliher et al. Importance of haploidy in plant breeding haploids are valuable tools haploids when doubled produce true breeding lines purelines or inbreds perfect homozygotes accelerate plant breeding. Two methods are generally used to produce haploids. Maternal and paternal haploids can be generated through reciprocal crosses. The effect of cenh3 variation in the solanum genus on genome elimination is being studied in collaboration with the laboratory of roger chetelat. Haploid plants produced by centromeremediated genome. Genome elimination in arabidopsis thaliana can be caused by parental cenh3 differences. Haploid plants produced by centromere mediated genome elimination ravi, m. We have also exploited centromere mediated genome elimination to convert a natural tetraploid arabidopsis into a diploid, reducing its ploidy to simplify breeding. Wa1 crosses contained only wa1 genetic markers table. Frontiers using centromere mediated genome elimination.
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